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ht1 buffer  (Illumina Inc)


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    Structured Review

    Illumina Inc ht1 buffer
    Ht1 Buffer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 471 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ht1+buffer/HT1+Buffer/bio_rxiv__64898__2026__10__07__757359-31-5-26
    Average 96 stars, based on 471 article reviews
    ht1 buffer - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Sequencing:

    Article Title: Methods of detecting and treating multiple system atrophy
    Article Snippet: The final library was sequenced according to the TempO-Seq Assay User Guide (version 2.0, 50 bp read1×9 bp index1×9 bp index2) on the NextSeq 500/550 High Output v2 kit (Illumina, 75 cycles, #FC-404-2005) on a NextSeq 500 (Illumina), at a final concentration of 1.6 pM with 2% PhiX. .. For sequencing, the BioSpyder TempO-Seq Custom Index 1 Sequencing Primer was used at 0.3 μM final concentration diluted in HT1 buffer (illumina, see nextseq-custom-primers-guide-15057456-01). .. Final library concentration was determined via the mean of three replicates using the Qubit dsDNA BR Assay (Invitrogen, #Q32853) on a Qubit 2 Fluorometer (Invitrogen).

    Article Title: Exposure to known and emerging groundwater contaminants significantly alters poultry microbiome and metabolome.
    Article Snippet: Final concentrations were verified using a KAPA library quantification kit (Kapa Biosys tems, Wilmington, MA, USA) and a Qubit 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). .. Next, the final library was diluted to 20 nM with HT1 buffer and PhiX control v3 (20%, vol/vol), and 600 μL was loaded onto a MiSeq v2 (500 cycles) reagent cartridge (Illumina, San Diego, CA, USA) to begin the sequencing run. .. The raw Illumina amplicon sequence data were uploaded to the BaseSpace website (Illumina, San Diego, CA, USA) to assess sequence run quality and completion.

    Concentration Assay:

    Article Title: Methods of detecting and treating multiple system atrophy
    Article Snippet: The final library was sequenced according to the TempO-Seq Assay User Guide (version 2.0, 50 bp read1×9 bp index1×9 bp index2) on the NextSeq 500/550 High Output v2 kit (Illumina, 75 cycles, #FC-404-2005) on a NextSeq 500 (Illumina), at a final concentration of 1.6 pM with 2% PhiX. .. For sequencing, the BioSpyder TempO-Seq Custom Index 1 Sequencing Primer was used at 0.3 μM final concentration diluted in HT1 buffer (illumina, see nextseq-custom-primers-guide-15057456-01). .. Final library concentration was determined via the mean of three replicates using the Qubit dsDNA BR Assay (Invitrogen, #Q32853) on a Qubit 2 Fluorometer (Invitrogen).

    Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
    Article Snippet: Libraries were pooled and denatured according to the Illumina Denature and Dilute Libraries Guide for the NextSeq System (Illumina Part #15048776). .. The denatured libraries were loaded onto an Illumina NextSeq 550 v2.5 Mid 150 output reagent cartridge AR TI CL E IN P RE SS (Illumina #20024904) at a final concentration of 1.5 pM in HT1 buffer, with 1% PhiX control library v3 (Illumina #FC-110-3001) added. ..

    Article Title: Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community
    Article Snippet: 370 bp) were purified using a 2% E-Gel Size Select agarose gel (Invitrogen, Carlsbad, CA). .. The concentration of the size-selected libraries was measured using a Qubit dsDNA HS Assay Kit and a Qubit fluorometer (Life Technologies, Carlsbad, CA), diluted to 12.0 pM with HT1 buffer (Illumina), and sequenced on the MiSeq platform (Illumina, San Diego, CA) using a MiSeq v2 Reagent Kit, 300 cycles (Illumina) with a PhiX Control v3 (Illumina, San Diego, CA) spike-in (expected at 5%), following the manufacturer’s protocol. ..

    Article Title: Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community.
    Article Snippet: 370 bp) were purified using a 2% E-Gel Size Select agarose gel (Invitrogen, Carlsbad, CA). .. The concentration of the size-selected libraries was measured using a Qubit dsDNA HS Assay Kit and a Qubit fluorometer (Life Technologies, Carlsbad, CA), diluted to 12.0 pM with HT1 buffer (Illumina), and sequenced on the MiSeq platform (Illumina, San Diego, CA) using a MiSeq v2 Reagent Kit, 300 cycles (Illumina) with a PhiX Control v3 (Illumina, San Diego, CA) spike-in (expected at 5%), following the manufacturer’s protocol. ..

    Control:

    Article Title: Exposure to known and emerging groundwater contaminants significantly alters poultry microbiome and metabolome.
    Article Snippet: Final concentrations were verified using a KAPA library quantification kit (Kapa Biosys tems, Wilmington, MA, USA) and a Qubit 2.0 Fluorometer (Invitrogen, Waltham, MA, USA). .. Next, the final library was diluted to 20 nM with HT1 buffer and PhiX control v3 (20%, vol/vol), and 600 μL was loaded onto a MiSeq v2 (500 cycles) reagent cartridge (Illumina, San Diego, CA, USA) to begin the sequencing run. .. The raw Illumina amplicon sequence data were uploaded to the BaseSpace website (Illumina, San Diego, CA, USA) to assess sequence run quality and completion.

    Article Title: IL-17RA signaling promotes the dedifferentiation of Paneth progenitors through ADAM17 to regenerate gut epithelium post-irradiation.
    Article Snippet: Libraries were pooled and denatured according to the Illumina Denature and Dilute Libraries Guide for the NextSeq System (Illumina Part #15048776). .. The denatured libraries were loaded onto an Illumina NextSeq 550 v2.5 Mid 150 output reagent cartridge AR TI CL E IN P RE SS (Illumina #20024904) at a final concentration of 1.5 pM in HT1 buffer, with 1% PhiX control library v3 (Illumina #FC-110-3001) added. ..

    Article Title: Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community
    Article Snippet: 370 bp) were purified using a 2% E-Gel Size Select agarose gel (Invitrogen, Carlsbad, CA). .. The concentration of the size-selected libraries was measured using a Qubit dsDNA HS Assay Kit and a Qubit fluorometer (Life Technologies, Carlsbad, CA), diluted to 12.0 pM with HT1 buffer (Illumina), and sequenced on the MiSeq platform (Illumina, San Diego, CA) using a MiSeq v2 Reagent Kit, 300 cycles (Illumina) with a PhiX Control v3 (Illumina, San Diego, CA) spike-in (expected at 5%), following the manufacturer’s protocol. ..

    Article Title: Large-scale environmental DNA survey reveals niche axes of a regional coastal fish community.
    Article Snippet: 370 bp) were purified using a 2% E-Gel Size Select agarose gel (Invitrogen, Carlsbad, CA). .. The concentration of the size-selected libraries was measured using a Qubit dsDNA HS Assay Kit and a Qubit fluorometer (Life Technologies, Carlsbad, CA), diluted to 12.0 pM with HT1 buffer (Illumina), and sequenced on the MiSeq platform (Illumina, San Diego, CA) using a MiSeq v2 Reagent Kit, 300 cycles (Illumina) with a PhiX Control v3 (Illumina, San Diego, CA) spike-in (expected at 5%), following the manufacturer’s protocol. ..



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